Scientists unraveling the molecular details of DNA recombination regulation

How DNA recombinational repair is regulated?

A molecular model for regulating Rad51 nucleoprotein filament formation by the Swi5-Sfr1 complex. Swi5–Sfr1 stabilizes Rad51 on ssDNA primarily by preventing its dissociation. This stabilization effect leads to a stable nucleating cluster formation and a reduction in filament disassembly. Red half-arrows indicate the kinetic steps affected by SSwi5–Sfr1.
A molecular model for regulating Rad51 nucleoprotein filament formation by the Swi5-Sfr1 complex. Swi5–Sfr1 stabilizes Rad51 on ssDNA primarily by preventing its dissociation. This stabilization effect leads to a stable nucleating cluster formation and a reduction in filament disassembly. Red half-arrows indicate the kinetic steps affected by SSwi5–Sfr1.

A worldwide group from National Taiwan University (NTU) and Tokyo Tech distinguished how DNA recombinational repair is directed in an ongoing work distributed in Proceedings of the National Academy of Sciences of the United States of America (PNAS).

To repair damaged DNA, recombinases tie to DNA with the end goal to start the repair work. Be that as it may, the recombinase-DNA complex must be adequately steady to do the work. Researchers from NTU and Tokyo Tech cooperate to recognize how another protein settles this recombinase-DNA complex to proficiently continue to appropriate DNA repair.

Prof. Hiroshi Iwasaki in Tokyo Tech said, “In addition to the beautiful science we did, this work stands as a model for the tremendous collaboration among biophysicists, biochemists and molecular biologists in Taiwan and Japan.”

Prof. Hung-Wen Li at NTU Chemistry Department said, “Watching the dynamics of these individual protein-DNA complex in real-time really offers information that is typically not available in conventional average-based experiments. Defects in DNA recombination are implicated in cancers. With this work, we now are in a position to design better strategies to battle DNA damages. This is just the beginning of the more exciting science in our joint adventure. It is fun and rewarding.”