Exciting plant vacuoles

Shedding new light on plant communication via electrical signals.


Many plant processes similar to the humans: Cells and tissues in grain plants, including maize also communicate through electrical signals. The shape and frequency of these signals tell the plants many different things including allowing them to respond to heat and cold, excessive light intensities or insect pests.

In other words, electrical signals are vital to passing information. A new study has shed light on plant communication using electrical signals, suggesting that the TPC1 ion channel contributes to plant excitability.

The function of this TPC1 channel has been previously unknown. This discovery may pave the way to breed plants that are more resistant to pests, heat or drought in the long run – properties that are crucial in the face of climate change.

The channel ion was primarily discovered in the mid of mid-1980s. Over the years, it has been studied more.

This new study fills another knowledge gap.

The cell membranes of plants were already known to be excitable; the scientists have now proved that this also applies to the membrane which encloses the central vacuole of the plant cells – and TPC1 plays a key role here as well. The vacuole is an enclosed compartment filled with an aqueous solution that can take up as much as 90 percent of the volume of a plant cell. Its main purpose is that of a pantry for storing nutrients.

How do electrical signals in the membrane of the vacuole develop? When a leaf is wounded, a calcium wave is triggered in the injured leaf in addition to the electrical signal. The two signals are mutually reinforcing, allowing the signal to propagate throughout the plant.

In plants with lack of TPC1, the calcium waves travel slowly.

Hedrich said, “This finding led us to study the properties of the vacuole using the patch clamp technique. It turned out that vacuoles lacking TPC1 can be excited, neither by an electric charge nor by a calcium boost. In a hyperactive mutant of TPC1, however, the excitation was sustained. “This technique and other analyses have enabled us to mathematically model vacuolar behavior and predict yet unknown properties of the vacuole channel.”

“Our discovery is also of interest in medical research. This is because relatives of the TPC1 channel have also been identified in humans. But it is not fully known yet which role the channel plays in the tiny membrane vesicles, the endosomes of our cells. Therefore, medical researchers are also looking for common features in the structure and function of TPC1 in plants and humans.”

JMU professor says, “To provide answers, US scientists conducted X-ray structural analyses for our patch clamp measurements to determine the molecular blueprint underlying the channel function. This makes TPC1 one of the best understood, voltage-dependent ion channels.”

TPC1 consists of two identical subunits. When they assemble to form a pair, a complex is created that has an ion channel at its center which response to voltage and calcium ions. There is a calcium receptor in the cytoplasmic end of TPC1 to activate the channel and another receptor on the inside of the vacuole. If the internal calcium concentration becomes too high, the channel is blocked and the vacuole loses its excitability.

TPC1 in evolution and climate change

“We also asked ourselves when the calcium-dependent TPC1 functions occurred in plants for the first time,” Hedrich says. Mosses were evidently the first to do so. “Now we aim to find out whether TPC1 is also responsible for the excitability in early ancestors of our crops and whether implanting alga or moss TPC1 is capable of healing the functional failure in mutants of modern crop plants.”

Moreover, the researchers discovered that there are plant families whose members exhibit significant differences in the development of the individual channel functions. They now want to understand the reason for these differences at the molecular level. Moreover, they intend to check whether the minor differences facilitate plant stress adaptation.

Scientists presented their findings in the renowned journal Nature Communications.

- Advertisement -

Latest Updates